Combination of high-performance thin-layer chromatography and liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry analysis: a promising analytical tool for discrimination between oleo-gum resin of raw and purified Commiphora wightii

Guggulu (Commiphora wightii oleo-gum resin) is exported in more than 42 countries including developed like the United Kingdom and States of America. The demand for guggulu its production. Various purification processes have been mentioned Ayurvedic classical literature which are not explored well yet, so it impossible to discriminate between purified raw guggulu. In present study, an effort was made where by using cow urine changes were analyzed high-performance thin-layer chromatography (HPTLC) liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry (LC–QTOF–MS/MS). HPTLC analysis, three new bands (RF 0.31, 0.68, 0.74) twelve other observed marker compounds guggulsterone E 0.81) Z 0.85) at 254 nm. LC–QTOF–MS/MS two peaks Rt 5.00 16.21 min urine-purified After purification, all resolute shows effects purification. Overall, LC–MS/MS metabolites, viz., 5-(13′Z-nonadecenyl)resorcinol (Rt 8.31), 8β-hydroxy-3,20-dioxopregn-4,6-diene 10.33), 12.99), 13.28), guggulsterol I = 15.19), mangiferolic acid 15.27), 20(S),21-epoxy-3-oxocholest-4-ene 15.75), II 16.23) identified. study reports first time a comparative analysis advanced analytical tools LC–QTOF–MS/MS. This may be helpful standardization quality control used various formulations